Table 2.
Primers and probes for real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis. Assay identification (ID), accession number, assay location, amplicon size and exon boundaries are listed (GJA1, Cx43; GJB1, Cx32; GJB2, Cx26; ACTB, actin beta; B2M, beta-2-microglobulin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HMBS, hydroxymethylbilane synthase; UBC, ubiquitin C).
| Gene Symbol | Assay ID | Accession Number | Assay Location | Amplicon Size (Base Pairs) | Exon Boundary |
|---|---|---|---|---|---|
| GJA1 ( Cx43 ) | Hs00748445-s1 | NM_000165.4 | 1031 | 142 | 2 |
| GJB1 ( Cx32 ) | Hs00939759-s1 |
NM_000166.5 NM_001097642.2 |
1547 1496 |
63 | 2 |
| GJB2 ( Cx26 ) | Hs00269615-s1 | NM_004004.5 | 715 | 123 | 2 |
| ACTB | Hs01060665-g1 | NM_001101.3 | 208 | 63 | 2–3 |
| B2M | Hs00187842-m1 | NM_004048.2 | 134 | 64 | 1–2 |
| GAPDH | Hs02786624-g1 |
NM_001256799.2 NM_001289745.1 NM_001289746.1 NM_002046.5 |
870 928 822 836 |
157 | 7 8 7 8 |
| HMBS | Hs00609296-g1 |
NM_000190.3 NM_001024382.1 NM_001258208.1 NM_001258209.1 |
1070 972 950 1041 |
69 | 13–14 13–14 12–13 12–13 |
| UBC | Hs01871556-s1 | M26880.1 | 2173 | 135 | – |