Table 1.
Transcript | Forward/ Reverse |
Primer Sequence | Annealing Temp. (°C) |
---|---|---|---|
MITF | F | 5’-GGTGGATGGGATAAGGGAAAG-3’ | 54.9 |
R | 5’-AGGACCTTGAAAACCGACAG-3’ | 55.1 | |
TRP-1 | F | 5’-GGTCTCCCTACATTTCCAGC-3’ | 55 |
R | 5’-AGCCCCAACTCTGTCTTTTC-3’ | 55.1 | |
TRP-2 | F | 5’-GGAAGGAGTGAGCCAAGTTATG-3’ | 54.9 |
R | 5’-TCCAGAAGTTTGACAGCCC-3’ | 55.4 | |
Tyrosinase | F | 5’-GGGTTTTGGCTTTGTCATGG-3’ | 55.2 |
R | 5’-CTAACTTACTCAGCCCAGCATC-3’ | 54.9 | |
GAPDH | F | 5′-GCCAAGGTCATCCATGACAAC-3′ | 59.9 |
R | 5′-GTCCACCACCCTGTTGCTGTA-3′ | 56.4 |