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. 2021 Oct 29;13(11):3894. doi: 10.3390/nu13113894

Table 1.

List of primers.

Transcript Forward/
Reverse
Primer Sequence Annealing Temp.
(°C)
MITF F 5’-GGTGGATGGGATAAGGGAAAG-3’ 54.9
R 5’-AGGACCTTGAAAACCGACAG-3’ 55.1
TRP-1 F 5’-GGTCTCCCTACATTTCCAGC-3’ 55
R 5’-AGCCCCAACTCTGTCTTTTC-3’ 55.1
TRP-2 F 5’-GGAAGGAGTGAGCCAAGTTATG-3’ 54.9
R 5’-TCCAGAAGTTTGACAGCCC-3’ 55.4
Tyrosinase F 5’-GGGTTTTGGCTTTGTCATGG-3’ 55.2
R 5’-CTAACTTACTCAGCCCAGCATC-3’ 54.9
GAPDH F 5′-GCCAAGGTCATCCATGACAAC-3′ 59.9
R 5′-GTCCACCACCCTGTTGCTGTA-3′ 56.4