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. 2021 Nov 18;22(22):12438. doi: 10.3390/ijms222212438

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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DRM cells have an increased migration potential. The wound healing assay indicated the area of wound closure in 22.5 nM, 50 nM, 100 nM DRM cells, and the parental cells which were 47%, 22%, 15%, and 58%, respectively. The cells were grown with 100% confluence and a wound was created with the use of a 1 mL tip. (A) The migratory effect of the cells was analyzed with the photograph of the etched area with the mentioned durations. (B) The area of the cell migration was measured with the use of ImageJ software and graphed. The values expressed as mean ± standard deviation (SD) (n = 5) ((* p < 0.05 vs. prental cells;, ** p < 0.001 vs. parental cells). DRM: Doxorubicin-resistant MDA-MB-231.