Figure 7.

DRM cells showed high cancer stem cells, high proliferation, and high EMT phenotype. Western blot analysis of markers for (A) cancer stem cells, (B) proliferation (C) EMT phenotype related proteins, and (D) Gelatin zymography of MMP proteins. Cells were seeded with a seeding density of 5 × 10⁴ cells and grown for 72 h. The control cells were left untreated. The whole-cell protein lysate was prepared, and 30 µg of proteins were resolved in SDS-polyacrylamide gels. Western blots were confirmed by three independent experiments. DRM: Doxorubicin-resistant MDA-MB-231.