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DRM cells showed high EGFR activity. Western blot analysis of EGFR and the downstream signal, Akt and ERK. Cells were seeded with a seeding density of 5 × 104 cells and grown for 72 h. The control cells were left untreated. The whole-cell protein lysate was prepared, and 30 µg of proteins were resolved in SDS-polyacrylamide gels. Western blots were confirmed by three independent experiments. DRM: Doxorubicin-resistant MDA-MB-231.
