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. 2021 Nov 18;22(22):12438. doi: 10.3390/ijms222212438

Figure 9.

Figure 9

Autocrine signaling in maintaining DRM cells. The parental cells were grown in resistant cell media showed an almost similar pattern of DRM cells in terms of the expression of CSC and EMT phenotype. Parental- MDA-MB-231 cells were grown in Doxorubicin-resistant cells grown media. (A) Morphological analysis of Parental- MDA-MB-231 cells, Parental- MDA-MB-231 cells grown in Doxorubicin resistant cells grown media. (B) Western blot analysis of autocrine signaling by analyzing the cancer stem cell markers and EMT phenotype. Results were confirmed by three independent experiments. DRM: Doxorubicin-resistant MDA-MB-231.