Figure 5.

Experimental setups for experiments in aquaria at atmospheric pressure. Perspective (a) and birds eye (b) views of the experimental setup for two-choice experiments on single P. elegans and M. fortunata. The shrimp was placed in the aquarium (P. elegans, room temperature; M. fortunata, 10 °C) and left to explore for 5 min. Two gauze bags (one control (C), one stimulus (S)) were introduced on each side of the tank. The shrimp were observed for 5 min. Perspective (c) and birds eye (d) views of the experimental setup for multiple-choice experiments on single P. elegans and M. fortunata. The shrimp was placed in the aquarium (P. elegans, room temperature; M. fortunata, 10 °C) after the introduction of four tubes containing agarose gels (three controls (C), one stimulus (S)) in the corners of the tank. The behavior of the shrimp was recorded for 30 min using a camera placed above the aquarium. Perspective (e) and birds eye (f) views of the experimental setup for two-choice experiments on a batch of M. fortunata. The experiments were conducted on several individuals in rearing tanks at 9 °C containing a heating thermostat (H) set at 25 °C. Two gels (one control (C), one stimulus (S)) were introduced on each side of the tank. The shrimp were observed for 30 min. Perspective (g) and birds eye (h) views of the experimental setup for experiments of choice between ON and OFF thermostats on a batch of P. elegans and M. fortunata. The experiments were conducted in rearing tanks at 9 °C containing several individuals of P. elegans or M. fortunata. Two temperature thermostats (one ON, the other OFF) were placed on the lateral sides of the tank, close to the surface. The number of shrimp positions on each thermostat was counted for 180 min, and then after one night. Arrows indicate the inlet and outlet of circulating seawater (SW).