| Antibodies |
| Mouse monoclonal anti-Actin (C4) |
Millipore |
Cat# MAB1501, RRID: AB_2223041 |
| Mouse monoclonal anti-Actin, alpha Smooth Muscle (clone 1A4) (Acta2) |
Sigma-Aldrich |
Cat# A2547, RRID: AB_476701 |
| Mouse monoclonal anti-EDA-Fibronectin (IST-9) |
Abcam |
Cat# ab6328, RRID: AB_305428 |
| Rabbit monoclonal anti-GAPDH (D16H11) |
Cell Signaling Technology |
Cat# 5174, RRID: AB_10622025 |
| Mouse monoclonal anti-GFP |
Abcam |
Cat# ab1218, RRID: AB_298911 |
| Mouse monoclonal anti-FLAG (M2) |
Sigma-Aldrich |
Cat# F1804, RRID: AB_262044 |
| Rabbit polyclonal anti-Myosin Light Chain (phospho S20) |
Abcam |
Cat# ab2480 RRID: AB_303094 |
| Rabbit polyclonal anti-Phospho-LATS1 (Thr1079) |
Cell Signaling Technologies |
Cat# 9159S, RRID: AB_10549072 |
| Rabbit monoclonal anti-Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) |
Cell Signaling Technologies |
Cat#4511,RRID:AB_2139682 |
| Rabbit monoclonal anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) |
Cell Signaling Technologies |
Cat# 4370, RRID: AB_2315112 |
| Rabbit monoclonal anti-p44/42 MAPK (Erk1/2) (137F5) |
Cell Signaling Technologies |
Cat# 4695 RRID:AB_390779 |
| Rabbit monoclonal anti-Phospho-Smad2 (Ser465/467) (138D4) |
Cell Signaling Technologies |
Cat# 3108, RRID:AB_490941 |
| Rabbit monoclonal anti-Phospho-Smad3 (Ser423/425) (C25A9) |
Cell Signaling Technologies |
Cat# 9520, RRID:AB_2193207 |
| Rabbit polyclonal anti-Phospho-YAP (Ser127) |
Cell Signaling Technologies |
Cat# 4911S, RRID:AB_2218913 |
| Rabbit monoclonal anti-Phospho-YAP (Ser397) (D1E7Y) |
Cell Signaling Technologies |
Cat# 13619, RRID:AB_2650554 |
| Rabbit monoclonal anti-Smad2 (D43B4) |
Cell Signaling Technologies |
Cat# 5339, RRID:AB_10626777 |
| Rabbit monoclonal anti- Smad3 (C67H9) |
Cell Signaling Technologies |
Cat# 9523, RRID:AB_2193182 |
| Rabbit polyclonal anti-Smooth Muscle Myosin Heavy Chain (Myh11) |
Biomedical Technologies |
Cat# BT-562, RRID:AB_10013421 |
| Rabbit polyclonal anti-TAZ (V386) |
Cell Signaling Technologies |
Cat# 4883S, RRID:AB_1904158 |
| Mouse monoclonal anti-Vinculin |
Sigma-Aldrich |
Cat# V9131, RRID:AB_477629 |
| Mouse monoclonal anti-YAP1 |
Abcam |
Cat# ab56701, RRID:AB_2219140 |
| Rabbit polyclonal anti-YAP (H-125) |
Santa Cruz |
Cat# sc-15407, RRID:AB_2273277 |
| Goat polyclonal anti-Mouse IgG (H+L), HRP |
Jackson ImmunoResearch |
Cat#115-035-166, RRID:AB_2338511 |
| Rabbit monoclonal anti-Ctgf (D8Z8U) |
Cell Signaling Technologies |
Cat# 86641T RRID:AB_2800085 |
| Rabbit monoclonal anti-Phospho-AKT (Thr308) (244F9) |
Cell Signaling Technologies |
Cat# 4056 RRID:AB_331163 |
| Rabbit Monoclonal anti-Phospho-AKT (S473) (D9E) XP |
Cell Signaling Technologies |
Cat# 4060 |
| Mouse monoclonal anti-AKT (40D4) |
Cell Signaling Technologies |
Cat# 2920 RRID:AB_10694382 |
| Goat polyclonal anti-Rabbit IgG (H+L), HRP |
Jackson ImmunoResearch |
Cat# 111-035-003, RRID:AB_2313567 |
| Goat polyclonal anti-Mouse IgG1 Secondary Antibody, Alexa Fluor 488 conjugate |
Thermo Fisher Scientific |
Cat# A-21121, RRID:AB_2535764 |
| Goat polyclonal anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher Scientific |
Cat# A-21131, RRID:AB_2535771 |
| Goat polyclonal anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 |
Thermo Fisher Scientific |
Cat# A-21135, RRID:AB_2535774 |
| Goat polyclonal anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher Scientific |
Cat# A-11008, RRID:AB_143165 |
| Goat polyclonal anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 |
Thermo Fisher Scientific |
Cat# A-11012, RRID:AB_2534079 |
| IRDye® 680RD Goat anti-Mouse IgG (H+L) |
LI-COR Biosciences |
Cat# 925-68070, RRID:AB_2651128 |
| IRDye® 800CW Goat anti-Rabbit IgG (H+L) |
LI-COR Biosciences |
Cat# 925-32211, RRID:AB_2651127 |
| Bacterial and Virus Strains |
| pInducer20_Yap1S127A-Flag |
Vector Builder (Song et al., 2014) |
N/A |
| pLV[Exp]-Puro-CMV>EGFP |
Vector Builder |
VB900088-2243bzq |
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| Chemicals, Peptides, and Recombinant Proteins |
| Dextran, Texas Red®, 70,000 MW |
Thermo-Scientific |
Cat#D1864 |
| Okadaic acid, Prorocentrum concavum
|
Sigma-Aldrich |
Cat# O7885 CAS: 155751-72-7 |
| Phalloidin CF™594 |
Biotium |
Cat#00045 |
| Recombinant Human TGFβ1 |
R&D Systems |
Cat#240-B-002 Accession # P01137
|
| Tautomycetin |
Tocris |
Cat# 2305 CAS: 119757-73-2 |
| Verteporfin |
United States Pharmacopeia |
Cat# 1711461 USP CAS: 129497-78-5 |
| Critical Commercial Assays |
| Deposited Data |
|
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| Experimental Models: Cell Lines |
|
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Acomys: A. C_E_M20 |
This Study |
N/A |
|
Acomys: A. C_E_M20_ hYap-GFP |
This Study |
N/A |
|
Acomys: A. C_E_N=6 |
This Study |
N/A |
| Mouse: M.M_E_CD1 |
This Study |
N/A |
| Human: SK5 Dermal |
Gift of Elaine Raines (Gronwald et al., 1988) |
RRID: CVCL_5H87 |
| Human: AB HASFB or AbdSK = Human Abdominal skin |
Promocell |
C-12352 |
| Human: AO HAFB = Human Aortic fibroblasts |
CellBiologics |
H-6075 |
| Human: CO HCFB =Human Coronary fibroblasts |
CellBiologics |
H-6049 |
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| Experimental Models: Organisms/Strains |
|
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|
Acomys cahirinus: Egyptian Spiny Mouse |
This study |
N/A |
|
Mus musculus: Crl:CD1(ICR) |
Charles River |
RRID:IMSR_CRL:22 |
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| Recombinant DNA |
|
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| pEGFP-C3-hYAP2 |
Gift of Marius Sudol (Basu et al., 2003) |
Addgene: #17844 |
| Software and Algorithms |
|
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| Fiji (Image J) |
http://fiji.sc/
|
N/A |
| Imaris 7.4 |
Bitplane |
N/A |
| Image Studio Light V5.2 |
https://www.licor.com/bio
|
N/A |
| VS-Desktop |
Olympus |
N/A |
| Prism7.03 |
Graph Pad |
N/A |
| Clustal O |
www.ebi.ac.uk/Tools/msa/clustalo/
|
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| NetPhos 3.1 |
(http://www.cbs.dtu.dk/services/NetPhos/) |
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| Other |
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