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. 2021 Nov 13;10(11):3153. doi: 10.3390/cells10113153

Figure 5.

Figure 5

Assessment of GILZ expression levels in skin DC subsets in the context of HPV-associated chronic inflammation. (a) Representative images for hematoxylin and eosin coloration of ear skin sections from HPV-transgenic mice (HPV) or their littermate controls (WT). Scale bar = 100 µm. (b) Epidermis and dermis ear thickness expressed as mean +/− SEM. At least 10 measures were performed for each sample. Data are from n=11 WT and 14 HPV mice for the epidermis and n = 2 WT and n = 6 HPV mice for the dermis. (c,d) Flow cytometry analysis of skin LCs, CD103− cDC1, CD103+ cDC1, cDC2 and DN DCs. (c) Gating strategy for DC subsets identification among alive single cells after exclusion of CD3+ T lymphocytes. (d) GILZ expression levels in skin DC subsets. Data were normalized relative to WT LCs and are expressed as median with interquartile range of normalized delta MFIs. Open symbols represent the values for individual mice. Data are from 4 independent experiments with a total n = 9 WT mice and n = 11 HPV mice (3 male and 6 female WT mice; 3 male and 8 female mice; FVB/N background). Statistical analysis was performed using the non-parametric Mann–Whitney U test to compare GILZ levels in cells from WT and HPV mice. Significance was defined as: p < 0.0001 ****.