Assessment of GILZ expression levels in TIDC subsets. (a,b) Flow cytometry analysis of CD11b− CD103+ cDC1, CD11b+ CD103− cDC2, CD11b− CD103− (DN) DCs, CD11b+ CD103+ (double positive, DP) cells and pDCs. (a) Gating strategy for tumor DC subset identification among CD45+ alive single cells. (b) GILZ expression level, in tumor DC subsets. Data were normalized relative to cDC1 and are expressed as median with interquartile range of delta MFIs. Data are from 2 independent experiments with a total of n = 9 tumor-bearing mice (male mice; C57BL/6J background). Statistical analysis was performed using the Kruskal–Wallis test to compare the different groups. Significance was defined as: p < 0.05 *.