Table 1.
List of mAbs and fixable viability dyes used in the study.
| Antigen | Fluorochrome | Supplier | Clone | Dilution |
|---|---|---|---|---|
| CD3 | V500 | BD Biosciences | 500A2 | 1/100 or 1/200 |
| CD8α | APC-eFluor 780 | eBiosciences | 53-6.7 | 1/100 |
| CD11b | FITC | BD Biosciences | M1/70 | 1/200 |
| CD11b | PE-CF594 | BD Biosciences | M1/70 | 1/200 |
| CD11b | Super Bright 600 | eBiosciences | M1/70 | 1/200 |
| CD11c | eFluor 450 | eBiosciences | N418 | 1/100 |
| CD11c | PE-Cy7 | eBiosciences | N418 | 1/100 |
| CD16/CD32 | Purified | BD Biosciences | 2.4G2 | 1/50 |
| CD45 | APC-Cy7 | BD Biosciences | 30F11 | 1/200 |
| CD64 | PE-Tred | BioLegend | X54-5/731 | 1/200 |
| CD103 | APC-R700 | BD Biosciences | M290 | 1/100 |
| CD207 | eFluor 660 | eBiosciences | eBioRMUL.2 | 1/50 |
| PDCA1/CD317 | eFluor 450 | eBiosciences | eBio927 | 1/100 |
| MHC II | PerCP Vio700 | Miltenyi Biotec | M5/114.15.2 | 1/200 or 1/500 |
| GILZ | PE | eBiosciences | CFMKG15 | 1/250 |
| GILZ Isotype | PE | eBiosciences | EBR2A | 1/250 |
| Viability dye | eFluor 506 | eBiosciences | 1/1000 |