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. 2021 Nov 20;9(11):1365. doi: 10.3390/vaccines9111365

Figure 1.

Figure 1

Western blot analysis of plant-produced IL6RmAb (pIL6RmAb). IL6RmAb was purified from N. benthamiana leaves and separated on SDS-PAGE gels under reducing (A,C) or non-reducing (B) conditions. After transferring the proteins to onto PVDF membranes, a house radish peroxidase (HRP)-conjugated goat anti-human kappa chain antibody (A,B) or an HRP-conjugated goat anti-human gamma chain antibody (C) were used to detect light chain or heavy chain, respectively. Lane 1, protein sample (~25 µg) from non-infiltrated leaves; Lane 2, protein sample (1 µg) from leaves infiltrated with IL6RmAb-expressing construct; Lane 3, protein sample of purified human IgG (1 µg).