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Specific binding of pIL6RmAb to IL-6R. pIL6RmAb was serially diluted and incubated with IL-6R coated on microtiter plate. After incubation, pIL6RmAb bound to IL-6R was detected with an HRP-conjugated anti-human-gamma HC antibody. Dilutions of a generic human IgG (hIgG) were used as the IgG isotype negative control. The OD450 values (mean ± SD) from two independent experiments are presented.
