Figure 4.
In vitro inhibition of IL-6 signaling by pIL6RmAb. Serial dilutions of pIL6RmAb or the human IgG isotype negative control antibody (hIgG) were incubated with cells containing luciferase as the downstream reporter of IL-6/IL-6R signaling, prior to exposure to recombinant human IL-6. After incubation, luciferase-detecting reagent was added and the bioluminescent signal was measured. The ratio of pIL6RmAb-treated signal to no antibody-treated signal was plotted versus antibody concentration to elucidate the inhibitory activity of antibodies. IC50 was calculated using GraphPad Prism 9.2. Two independent experiments were performed, and error bars represent standard error of the mean.