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. 2021 Nov 19;22(22):12512. doi: 10.3390/ijms222212512

Figure 9.

Figure 9

Role of intracellular ROS in AKR1C1 and 1C3 mediating chemo-resistance in signet ring gastric cancer cells. (A) Intracellular ROS level in KATOIII and KATO/DDP cell lines after 1 h treated with cisplatin was detected by DCF-DA ROS assay. (B) KATOIII cells were treated with cisplatin with or without NAC for 48 h at 37 °C. Cell viability was assessed by using trypan blue cell exclusion assay. (C) Intracellular ROS level in KATO/DDP cells after treatment with cisplatin and ASP9521 or 5PBSA. KATO/DDP cells were pretreated with ASP9521 or 5PBSA at 10 µM for 3 h and added with cisplatin at 25 µM. Intracellular ROS was measured by DCF-DA ROS assay after 1 h of cisplatin treatment. (D) Cell viability of KATO/DDP cells treated with cisplatin and ASP9521 or 5PBSA with or without NAC. Data are presented as mean ± SD; * represents p < 0.05 and ** represents p < 0.01.