. 2021 Nov 20;22(22):12528. doi: 10.3390/ijms222212528

Table 2.

The intensity of MMP-13, MMP-8 and TIMP-2 immunoreaction in bone and articular tissue of female rat offspring following maternal exposure to 0, 60 and 90 mg/kg b.w. of fumonisins.

Dependent Variable	Region		FB (mg/kg b.w.)			p-Value	p-Level
Dependent Variable	Region		0	60	90	p-Value	Linear	Quadratic
MMP-13	growing plate	proliferative	116.66 ± 7.79 ^a	78.09 ± 1.87 ^b	98.92 ± 8.76 ^a,b	<0.001	0.026	0.002
	growing plate	hypertrophic	86.66 ± 1.86 ^a	111.41 ± 7.38 ^b	119.13 ± 7.43 ^b	0.005	0.002	0.003
	articular		150.76 ± 12.45 ^a	101.29 ± 3.27 ^b	86.97 ± 2.61 ^b	<0.001	<0.001	<0.001
	trabecular	osteocyte	190.59 ± 9.27 ^a	158.14 ± 7.42 ^b	139.62 ± 6.72 ^b	0.001	<0.001	0.002
	trabecular	matrix	158.23 ± 7.04 ^a	112.42 ± 4.86 ^b	92.23 ± 7.72 ^b	<0.001	<0.001	<0.001
	compact	osteocyte	184.21 ± 10.01 ^a	210.61 ± 5.77 ^a	116.11 ± 5.56 ^b	<0.001	<0.001	0.065
	compact	matrix	134.83 ± 8.40	152.05 ± 4.21	120.60 ± 1.42	0.054	0.282	0.253
MMP-8	growing plate	proliferative	93.27 ± 1.05 ^a	80.76 ± 3.91 ^b	77.28 ± 2.31 ^b	0.002	0.001	0.001
	growing plate	hypertrophic	125.69 ± 11.56	105.63 ± 4.74	104.15 ± 9.98	0.188	0.078	0.092
	articular		191.28 ± 11.99 ^a	117.59 ± 8.28 ^b	111.88 ± 5.04 ^b	<0.001	<0.001	<0.001
	trabecular	osteocyte	220.02 ± 12.91 ^a	178.91 ± 4.73 ^b	105.58 ± 9.40 ^c	<0.001	<0.001	<0.001
	trabecular	matrix	139.81 ± 3.09 ^a	99.48 ± 10.87 ^b	151.86 ± 14.32 ^a	0.009	0.912	0.002
	compact	osteocyte	174.57 ± 9.16 ^a	220.66 ± 3.99 ^b	128.16 ± 7.34 ^c	<0.001	0.009	0.045
	compact	matrix	125.95 ± 6.99	110.64 ± 4.06	118.98 ± 3.38	0.154	0.230	0.070
TIMP-2	growing plate	proliferative	242.35 ± 3.18 ^a	217.17 ± 5.67 ^b	88.21 ± 5.64 ^c	<0.001	<0.001	<0.001
	growing plate	hypertrophic	226.13 ± 3.68 ^a	185.12 ± 6.18 ^b	132.12 ± 5.08 ^c	0.001	<0.001	<0.001
	articular		166.55 ± 6.25	182.75 ± 2.57	193.23 ± 15.33	0.180	0.069	0.137
	trabecular	osteocyte	221.86 ± 5.93	205.72 ± 3.31	225.94 ± 1.84	0.003	0.994	0.039
	trabecular	matrix	151.75 ± 3.68	136.24 ± 0.97	139.78 ± 7.48	0.092	0.062	0.061
	compact	osteocyte	237.74 ± 3.68 ^a	180.51 ± 1.86 ^b	198.87 ± 3.99 ^c	<0.001	<0.001	<0.001
	compact	matrix	156.21 ± 6.70	140.79 ± 2.21	141.25 ± 5.25	0.081	0.055	0.221

Results are presented as mean ± SE with corresponding statistical analysis. FB—fumonisin, MMP-13—matrix metalloproteinase 13, MMP-8—matrix metalloproteinase 8, TIMP-2—metalloproteinase inhibitor. Statistically significant differences between groups (at p-value < 0.05) are indicated by ^a, ^b and ^c.