. 2021 Nov 20;22(22):12528. doi: 10.3390/ijms222212528

Table 3.

The intensity of MMP-13, MMP-8 and TIMP-2 immunoreaction in bone and articular tissue of male rat offspring following maternal exposure to 0, 60 and 90 mg/kg b.w. of fumonisins.

Dependent Variable	Region		FB (mg/kg b.w.)			p-Value	p-Level
Dependent Variable	Region		0	60	90	p-Value	Linear	Quadratic
MMP-13	growing plate	proliferative	89.99 ± 3.51	87.71 ± 1.93	94.59 ± 11.12	0.771	0.719	0.972
	growing plate	hypertrophic	155.85 ± 13.50	116.79 ± 10.17	141.59 ± 9.09	0.071	0.201	0.057
	articular		124.54 ± 8.22 ^a	81.79 ± 1.59 ^b	83.79 ± 3.25 ^b	<0.001	<0.001	<0.001
	trabecular	osteocyte	196.98 ± 4.73 ^a	156.76 ± 4.04 ^b	117.00 ± 1.33 ^c	<0.001	<0.001	0.960
	trabecular	matrix	168.63 ± 8.26 ^a	106.15 ± 9.95 ^b	111.16 ± 4.21 ^b	<0.001	<0.001	<0.001
	compact	osteocyte	202.95 ± 8.29 ^a	130.40 ± 12.72 ^b	128.04 ± 3.67 ^b	<0.001	<0.001	<0.001
	compact	matrix	121.41 ± 5.59 ^a	158.13 ± 4.11 ^b	110.34 ± 2.70 ^a	<0.001	0.789	0.001
MMP-8	growing plate	proliferative	113.24 ± 4.13	123.66 ± 1.33	126.96 ± 8.85	0.237	0.097	0.134
	growing plate	hypertrophic	201.27 ± 12.25	208.31 ± 5.99	172.15 ± 19.43	0.176	0.237	0.852
	articular		181.91 ± 9.48 ^a	118.87 ± 8.88 ^b	133.31 ± 6.81 ^b	<0.001	<0.001	<0.001
	trabecular	osteocyte	222.45 ± 3.89 ^a	175.96 ± 8.45 ^b	149.31 ± 1.68 ^c	<0.001	<0.001	<0.001
	trabecular	matrix	104.90 ± 5.67	123.29 ± 9.80	131.28 ± 9.49	0.114	0.048	0.074
	compact	osteocyte	206.23 ± 5.28 ^a	117.86 ± 7.97 ^b	208.96 ± 7.25 ^a	<0.001	0.105	<0.001
	compact	matrix	130.26 ± 7.47 ^a	88.12 ± 5.28 ^b	180.73 ± 6.57 ^c	<0.001	0.002	0.075
TIMP-2	growing plate	proliferative	246.78 ± 2.59 ^a	168.37 ± 10.77 ^b	169.25 ± 3.73 ^b	<0.001	<0.001	<0.001
	growing plate	hypertrophic	243.00 ± 2.21 ^a	94.88 ± 6.88 ^b	146.66 ± 2.10 ^c	<0.001	<0.001	<0.001
	articular		196.23 ± 2.65 ^a	147.51 ± 11.60 ^b	175.32 ± 10.45 ^a,b	0.007	0.041	0.003
	trabecular	osteocyte	243.27 ± 1.94 ^a	222.82 ± 5.58 ^b	158.03 ± 4.52 ^c	<0.001	<0.001	<0.001
	trabecular	matrix	153.18 ± 3.38 ^a	136.16 ± 1.62 ^b	110.27 ± 2.42 ^c	<0.001	<0.001	<0.001
	compact	osteocyte	193.98 ± 4.30	189.69 ± 5.23	193.03 ± 10.20	0.904	0.862	0.714
	compact	matrix	124.61 ± 5.50	125.30 ± 2.99	131.35 ± 8.04	0.679	0.480	0.749

Results are presented as mean ± SE with corresponding statistical analysis. FB—fumonisin, MMP-13—matrix metalloproteinase 13, MMP-8—matrix metalloproteinase 8, TIMP-2—metalloproteinase inhibitor 2. Statistically significant differences between groups (at p-value < 0.05) are indicated by ^a, ^b and ^c.