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. 2021 Nov 14;13(11):4070. doi: 10.3390/nu13114070

Figure 5.

Figure 5

The effect of Ica on mitochondrial permeability transition pore opening in Doxo-treated H9c2 cells. (A). Mitochondrial permeability transition pore (mPTP) opening was determined by immunofluorescence assay. Fluorescence was quantified using ImageJ® software by converting the intensity to a greyscale value based on the RGB color model. For each treatment, 30–40 cells were processed. Significant differences in mean fluorescence were found between all treated cells. (B). Confocal representative images of H9c2 cells treated as described above. Green staining showed calcein, blue staining showed Hoechst 33342 used for nucleic acid staining, red staining showed MitoTracker Red CMXRos used for mitochondria staining. Cell images were collected using a 40× confocal microscope objective. Data are expressed as mean ± SEM, *** p < 0.001 vs. Ctrl; °°° p < 0.001 vs. Ica 1 μM ^^^ p < 0.001 vs. Ica 5 μM; ### p < 0.001 vs. Doxo; Kruskal-Wallis test and Dunn’s multiple comparisons test (n = 3). Data is from three independent experiments.