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. 2021 Nov 14;13(11):4070. doi: 10.3390/nu13114070

Figure 6.

Figure 6

Cytofluorimetric evaluation of the effect of Ica against Doxo-induced apoptotic cell death. (A). H9c2 cells treated for 3 h with both Ica 1 μM and 5 μM showed no significant apoptotic or necrotic cell death. (B). Treatment with Doxo 1 μM for 24 h resulted in a detrimental increase in apoptotic cell death when compared to Ctrl cells. Conversely, pretreatment of H9c2 cells with Ica 1 μM and 5 μM significantly prevented apoptosis. (C) Representative cytofluorimetric dot plot. Data are expressed as means ± SEM.; ** p < 0.01 vs. Ctrl, ## p < 0.01 vs. Doxo; Mann Whitney test (n = 3). Data is for three independent experiments.