Figure 9.

The effect of Ica on the pro-autophagic markers Beclin-1 and LC3. Treatment with Doxo alone significantly increased the expression levels of Beclin-1 (A) and LC3II/LC3I ratio (C) in H9c2 cells. Pretreatment with Ica at both concentrations used inhibited the proautophagic pathway when compared to H9c2 cells treated with Doxo alone. (B,D) Representative images of western blot analysis. Three independent Western Blots were quantified by densitometry. The protein expression of Beclin-1 and LC3II/LC3I were normalized with respect to the corresponding tubulin or actin signals of the appropriate samples and expressed as a percentage. Data are expressed as mean ± S.E.M. ** p < 0.01 vs. Ctrl; ° p < 0,05 vs. Ica 1uM; ^ p < 0.05 vs. Ica 5 μM; # p < 0.05 vs. Doxo; ## p < 0.01 vs. Doxo; Mann Whitney test (n = 3).