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. 2021 Nov 17;26(22):6923. doi: 10.3390/molecules26226923

Figure 2.

Figure 2

Effects of quercetin (Q) and quercetin-penta acetate analogue (Q5) on macrophages (in vitro). Peritoneal exudate macrophages stimulated or not with LPS + INFγ were cultured in the presence or absence of compounds (20, 40 or 80 µM) or dexamethasone (20 µM). Cell viability was determined by the Alamar Blue method (A,D). Cell supernatant was collected after 24 h for nitrite quantification (B,E) or 4 h for TNF measurement (C,F). C- Group of untreated and unstimulated cells. C- Group of cells stimulated with LPS + INFγ. Values are represented by the mean ± standard deviation of the mean of nine determinations obtained from three independent experiments. *** p < 0.001 compared to stimulated and untreated cells, ** p < 0.01 compared to stimulated and untreated cells, # p < 0.05 compared to unstimulated and untreated cells and $ p < 0.05 compared to cells treated with dexamethasone.