Figure 2.

In Vitro activity of compound 62520 against A. baumannii ATCC 19606, A. nosocomialis ATCC 17903, and clinical CRAB isolates. (A) Bacteria were cultured with or without 2.8 μg/mL of compound 62520 for 24 h and bacterial growth was measured at OD₆₀₀. Data are presented as the mean ± SD of three independent experiments. (B) Bacteria were cultured with or without 2.8 μg/mL of compound 62520 for 24 h and then total RNA was extracted. The expression levels of ompA were analyzed by qPCR. Data are presented as the mean ± SD of the expression levels of ompA in compound 62520-treated bacteria relative to those of untreated bacteria. (C) Bacteria were cultured with or without 2.8 μg/mL of compound 62520 for 24 h. The biofilm mass was quantitated as the OD₅₇₀ normalized to OD₆₀₀. For both (B,C), data are presented as the mean ± SD of three independent experiments. * p < 0.05 compared with the untreated bacteria.