Table 4.

Comparison between HPLC, VICAM, and validated method I-ELISA.

Parameters	HPLC	VICAM	I-ELISA
Limit of detection	<0.008 ng/mL	<2 ng/mL	<1 ng/mL
Time of analysis	120 min	90 min	30 min
Cost of analysis	High	Moderate	Moderate
Use of organic solvents	Yes	Yes	No