FIG. 1.

Effects of hat1Δ and histone H3 single-lysine-to-arginine mutations on telomeric silencing. The indicated histone H3 alleles were introduced into UCC1111 (HAT1) and MPY1 (hat1Δ). Telomeric silencing was measured by spotting 10-fold serial dilutions of cells on synthetic complete plates (HC) and synthetic complete plates containing 5-FOA (HC+5-FOA) (Materials and Methods). Plates were photographed after 3 days of growth at 30°C. The relevant genotypes of the strains are indicated on the left. WT, wild type. Assays were performed on at least three separate colonies from each strain.