Figure 3.

MALDI MSI of Aspergillus fumigatus grown on agar (24 h at 37 °C), and influence of matrix application on sensitivity and spatial resolution. (a) A. fumigatus culture (optical image a0) using dry-coating application of a 1:1 mixture of DHB and CHCA (optical image a1 after matrix application and magnification a3); (b) A. fumigatus culture (optical image b0) using coating with a dispersed 1:1 mixture of DHB and CHCA in CHCl₃ (optical image b1 after matrix application and magnification b3). In both panels, the relative distribution (ion images a2 and b2 and their respective magnifications a4 and b4) and the average mass spectrum of dfTafC are depicted (m/z 891, [M + K]⁺) [26].