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. 2021 Nov 17;22(22):12393. doi: 10.3390/ijms222212393

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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3D MALDI FTICR MSI of soybean root nodule metabolism. (a) Building of tomography images from 2D images. (b) Microscopic anatomical regions imaged by characteristic compounds: co-localization of UDP-N-acetyl hexosamine with the infection zone, flavonoid glycoside with the inner cortex, and soyasaponin within the outer cortex of the soybean nodule. 3D distribution of (c) SAM and (d) heme B within the soybean root nodule. 3D distribution within soybean nodules of the (e) polyamine and (f) PC biosynthesis pathways involving SAM during biological nitrogen fixation [67].