Table 1.

List of molecules detected in different organisms using MALDI-MSI and their localizations.

Sample	Compartment	Class of Compound	Matrix	Technique	Reference
Dried agar slices	Microbial extracellular interface	Siderophores	DHB/CHCA (1:1)	MALDI TOF MSI and ion mobility	[26,30,37]
Piece of medium placed on MALDI plate	Interface of fungal colonies	Indol alkaloids	DHB and DHB/CHCA (1:1)	MALDI TOF MSI	[34]
Agar piece placed on MALDI plate	Fungal mycelium	Polyketide	Nanostructured silicon	MALDI TOF MSI on nanosilicone	[25]
Ant thorax section on ITO slide	Ant exoskeleton: propleural plate	Ergothioneine	DHB	MALDI Orbitrap MSI	[32]
Agar section on MALDI plate	Microbial extracellular interface	Quinolones rhamnolipids	DHB/CHCA (1:1)	MALDI FT ICR MSI	[40,41]
Agar section on MALDI plate	Chlamydospores	Cyclo-lipopeptide	DHB/CHCA (1:1)	MALDI MSI	[43]
Cryosection of sclerotia	Sclerotia	Alkaloids	DHB	MALDI MSI (Synapt G2 with ion mobility)	[44]
Dried colonies on the slide	Inhibitory zone at the microbial interface	Solanapyrones, disaccharides, isoflavones, and isoflavonoids	DHB	MALDI FT ICR MSI	[46]
Agar cut on ITO slide	Inhibitory zone	lipopeptaibol	DHB/CHCA (1:1)	MALDI MSI	[48]
Surface tissue section on MALDI plate	Pea pods	Aromatic compound	DHB	MALDI MSI with ion mobility	[74]
Leaf imprints on porous PTFE sheet	Rice root	Diterpenoids	Fe3O4 nanoparticles	MALDI MSI	[60]
Root on MALDI plate with nylon membrane	Root	Organic acids	CHCA	MALDI MSI Synapt G1	[61]
Lyophilized root on slide	Rhizosphere	Acyl sugars and steroidal glycoalkaloids	DHB	MALDI FT ICR MSI	[62]
PVDF membrane with roots on MALDI plate	Rhizosphere interface	Nucleobase, amino acid, and derivatives	DHB	MALDI FT ICR MSI	[63]
Root-colonized growth on ITO slide	Aerial roots/root biofilm coated	Cyclo-lipopeptides	CHCA	MALDI TOF MSI	[65,66]
Cryosection of root on ITO slide	Root nodule	Choline derivative, organic acid, and amino acids	Norharmane	3D MALDI FTICR MSI	[67]
Cryosection of nodules on plain glass microscope slide	Nodule	Amino acid, triterpenoid, phospholipid, disaccharide, nucleobases, vitamins	DHB and CHCA	AP MALDI MSI Orbitrap	[70,71]
Tissue covered with aluminum foil and embedded in paraffin. Microtome sections on ITO slide	Stem, leaf	flavonoids	DHB/CHCA (1:1)	MALDI TOF MSI	[75]
Cryosection of nodules on glass microscope slide	Nodule	Peptide	DHB and CHCA	MALDI Orbitrap MSI	[69]
Cryosections on ITO slides	Leaf epidermises, vascular bundles, roots, petiole collenchyma, root	Cyclotides	DHB	MALDI TOF MSI	[72]
Leaf fixed with aluminized tape on MALDI plate	Leaf	Stilbenoid and polyphenol	DHB	MALDI TOF MSI	[73]
Cryosections	Brain	Neuropeptide and tryptic peptides	CHCA	MALDI TOF MSI	[76,81]
Cryosections on frosted glass slides	Fly genitals	Pheromones and lipids	DHB	MALDI Orbitrap MSI	[27]
Cryosection on ITO slides	Hemolymph	Glucosinolate	CHCA	MALDI TOF MSI	[82]
Cryosections on ITO slides	Worm	Amino acid, sugars, and polyamine	2-MBT	MALDI TOF MSI	[89]
Cryosections	Rat brain	Phospholipids, neurotransmitters, nucleotides	DHB, nanostructure initiator, DAN, BNDM, and norharmane	MALDI TOF MSI MALDI Orbitrap MSI MALDI Orbitrap with ozonolysis	[92,93,94,95,100,102]
Cryosections	Rat testicles	Seminolipids, phospholipids, and steroids	CHCA, DHB, and AA. Derivatization for steroids.	MALDI TOF MSI MALDI FT ICR MSI iMScope QT	[105,106,108,109,110]
Cryosections	Rat kidney	Phospholipids and small-molecular-weight metabolites	DHB, benzoylpyridine CHCA, and DAN	AP-MALDI Orbitrap MSI MALDI timsTOF MSI MALDI TOF MSI	[112,113,114]