Table 3.

Summary of ChAdOx1 RVF in vivo immunogenicity.

Vaccine	ELISA Antigen	Time since Vaccination	n 2	Dose (IU)	Immunogenicity in Mice (Log Change) 1
					Liquid				Lyophilised
					−80 °C	4 °C	20 °C	30 °C	4 °C	20 °C	30 °C
ChAdOx1 RVF	Gc	1 month	24	1 × 105	0.0	−0.5	−0.3	−0.7	-	-	-
			24	1 × 106	0.0	0.1	0.1	0.1	-	-	-
			24	1 × 107	0.0	0.1	−0.3	−0.5	-	-	-
		12 months	18	1 × 105	0.0	0.1	−1.5	-	-	-	-
			18	1 × 106	0.0	−0.2	−1.1	-	-	-	-
			18	1 × 107	0.0	−0.2	−0.3	-	-	-	-
		16 months	18	1 × 105	-	-	-	-	0.0	−1.6	−1.6
			18	1 × 106	-	-	-	-	0.0	0.2	−1.4
			18	1 × 107	-	-	-	-	0.0	0.0	−0.3
	Gn	1 month	24	1 × 105	0.0	−0.6	0.1	−0.5	-	-	-
			24	1 × 106	0.0	0.7	0.6	−0.4	-	-	-
			24	1 × 107	0.0	0.1	0.1	−0.5	-	-	-
		12 months	18	1 × 105	0.0	−0.2	−1.7	-	-	-	-
			18	1 × 106	0.0	−0.3	−1.7	-	-	-	-
			18	1 × 107	0.0	0.0	−0.4	-	-	-	-
		16 months	18	1 × 105	-	-	-	-	U 3	U	U
			18	1 × 106	-	-	-	-	U	U	U
			18	1 × 107	-	-	-	-	0.0	−0.2	−1.4

¹ Median log change in antibody titre from six mice in each group. Log change for liquid formulations is in relation to vaccine stored at −80 °C. Log change for lyophilised formulations is in relation to vaccine stored at 4 °C. ² number of mice in total per row (for each temperature, n = 6). Gn and Gc ELISA responses were measured in the same mice, so they do not represent additional animals. ³ U = undetectable antibody response.