Table 2.
The main co-culture models (reproduced after Nishiguchi et al. [58], Aengenheister et al. [56], Blundell et al. [65]).
| Model | Sandwich Culture | Transwell Insert | Placenta-on-a-Chip System |
|---|---|---|---|
| Authors | Nishiguchi et al. 2019 [58] | Aengenheister et al., 2018 [56] | Blundell et al., 2018 [65] |
| http://creativecommons.org/licenses/by/4.0/, accessed on 10 November 2021 | |||
| Villous cytotrophoblasts | primary VCTs (third trimester) with collagen and laminin coating | BeWo b30 | BeWo b30 |
| Villous endothelial cells | human umbilical vein endothelial cells (HUVECs) with fibronectin and gelatin coating | microvascular human placental venous endothelial cell line (HPEC-A2) | human primary placental villous endothelial cells (HPVECs) |
| Villous mesenchymal fibroblasts | primary human villous mesenchymal fibroblasts (HVMFs) with fibronectin and gelatin coating | none | none |
| Technology | bottom-up approach using ECM (extracellular matrix) nanofilms | polycarbonate Transwell insert | upper and lower microchannels separated by a thin, semipermeable membrane |
| Description of the model |
|
|
|