Table 2.

The main co-culture models (reproduced after Nishiguchi et al. [58], Aengenheister et al. [56], Blundell et al. [65]).

Model	Sandwich Culture	Transwell Insert	Placenta-on-a-Chip System
Authors	Nishiguchi et al. 2019 [58]	Aengenheister et al., 2018 [56]	Blundell et al., 2018 [65]
		http://creativecommons.org/licenses/by/4.0/, accessed on 10 November 2021
Villous cytotrophoblasts	primary VCTs (third trimester) with collagen and laminin coating	BeWo b30	BeWo b30
Villous endothelial cells	human umbilical vein endothelial cells (HUVECs) with fibronectin and gelatin coating	microvascular human placental venous endothelial cell line (HPEC-A2)	human primary placental villous endothelial cells (HPVECs)
Villous mesenchymal fibroblasts	primary human villous mesenchymal fibroblasts (HVMFs) with fibronectin and gelatin coating	none	none
Technology	bottom-up approach using ECM (extracellular matrix) nanofilms	polycarbonate Transwell insert	upper and lower microchannels separated by a thin, semipermeable membrane
Description of the model