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. 2021 Nov 11;22(22):12195. doi: 10.3390/ijms222212195

Figure 3.

Figure 3

MACC1 activity is directly related to IGFBP2 expression, which interferes with platelet activation. The downregulation of IGFBP2 in the MACC1 KO variant of SW620 cells was indicated at the mRNA level by qPCR (A) and confirmed at the protein level by ELISA (B). ELISA data confirm the lower expression of IGFBP2 by the MACC1 KO variant of SW620 cells, either in supernatant (s. MACC1 Ctrl vs. s.MACC1 KO, left columns) and at a lower level in the cell lysate (right columns). (C) IGF-I is costimulatory for platelet activation and accelerates the effect of 7.5 µM TRAP-6 to induce platelet aggregation. (D) IGF-I is associated with platelets and found in platelet plasma or supernatant after activation, but not in considerable amounts in supernatant of SW620 cells. (E) Recombinant IGFBP2 is able to block concentration-dependently the activity of TRAP-6 to induce platelet aggregation and (F), significantly, the platelet ATP release. *** p < 0.001; **** p < 0.0001.