Skip to main content
. 2021 Oct 28;7(11):916. doi: 10.3390/jof7110916
Test Parameter CLSI M38 EUCAST E.Def 11.0
Glucose concentration in susceptibility testing medium (RPMI 1640 with L-glutamine and without sodium bicarbonate, pH = 7) 0.2% 2%
Cycloheximide and chloramphenicol supplementation of the inoculum #x2212; +
Inoculum type and concentration Conidia
1−3 × 103 cfu/mL
Microconidia
1−2.5 × 105 cfu/mL
Microdilution plates 96-well U-shaped 96-well flat-bottom
Incubation period and temperature for the microdilution plates 4 days at 35 °C 5 days at 25−28 °C
MIC reading method Visual Spectrophotometric
MIC reading endpoint MIC-1 (80% inhibition)
(Terbinafine, azoles, ciclopirox olamine, and griseofulvin)
MIC-2 (50% optic density reduction)
(Terbinafine, azoles, and amorolfine)