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Detection of HEV RNA in cell culture supernatant after (A) inoculation of PLC/PRF/5 cells with wild boars samples (1st passage) or (B) inoculation of the supernatants from 96 days post-infection (d p.i.) of the 1st passage on fresh PLC/PRF/5 cells (2nd passage). Supernatants of first and second passage were analyzed by RT-qPCR and the amounts are shown in log genome copies (gc)/mL. <LOD, below limit of detection.
