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Representative results demonstrating specificity of biotin switch assay. Lysates of the prefrontal cortex were incubated for 30 min with (or in the absence of) 200 μM S-nitrosoglutathione (GSNO), a NO donor. During the labeling step with or without the addition of sodium ascorbate (Na-Asc), biotinylation of the nascent thiols occurred. The sample treated with both GSNO and Na-Asc showed a strong signal compared to other control samples.
