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. 2021 Nov 17;14(11):1178. doi: 10.3390/ph14111178

Figure 4.

Figure 4

RIG-I is required for IFN-β production in response to ER stress. (A) Liver homogenates were prepared from mice after 12 weeks of treatment as described in Figure 1. The mRNA levels of sXBP-1 and tXBP-1 were determined by qRT–PCR. (B) Primary mouse hepatocytes isolated from WT or RIG-I KO mice were treated with tunicamycin (1 μg/mL) for 16 h. The mRNA levels of sXBP-1 and tXBP-1 were determined by qRT–PCR. The protein expression of RIG-I was determined by immunoblotting of the hepatocytes. The original immunoblotting is provided in Figure S3, Supplementary Materials. (C) Primary mouse hepatocytes isolated from WT or RIG-I KO mice were treated with tunicamycin (2 μg/mL) or thapsigargin (10 μM) for the indicated times. The mRNA levels of IFN-β were determined by qRT–PCR. (D) Bone marrow-derived macrophages from WT or RIG-I KO mice were treated with tunicamycin or thapsigargin for 24 h. The mRNA levels of IFN-β were determined by qRT–PCR. The values represent the means ± SEM (n = 3–5). * p < 0.05.