SpeI-generated macrorestriction fragments of the B. cepacia complex strain panel separated by PFGE. Digestion and separation of macrorestricted DNA were performed as described in the text, and restriction fragments were visualized after staining with ethidium bromide. Molecular size standards were run in the lanes labelled M, and the sizes of relevant marker bands (in kilobases) are shown on the left. Strains analyzed (see Table 1) in each lane are as follows: 1, ATCC 25416T; 2, ATCC 17759; 3, CEP509; 4, LMG 17997; 5, C5393; 6, LMG 13010T; 7, C1576; 8, CP-A1-1; 9, JTC; 10, C1962; 11, ATCC 17616; 12, 249-2; 13, J2315; 14, BC7; 15, K56-2; 16, C5424; 17, C6433; 18, C1394; 19, PC184; 20, CEP511; 21, J415; 22, ATCC 17765; 23, LMG 14294; 24, C7322; 25, LMG 14086; 26, LMG 18888; 27, PC259; 28, LMG 16232; 29, FC441; 30, LMG 10929T.