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. 2021 Nov 17;13(11):1944. doi: 10.3390/pharmaceutics13111944

Figure 1.

Figure 1

Affibody-mediated CAIX targeting on-chip. (A) Schematic representation of an affibody fused to mCherry. (B) Schematic representation of the microfluidic chip. (C,D) Confocal microscopy images of a tumor-on-a-chip after a one-hour incubation of CAIX-positive SK-RC-52 (C) or CAIX-negative SKRC-17 cells (D) with the anti-CAIX affibody-mCherry fusion or the ZTAQ affibody-mCherry fusion, which was used as non-binding control. (E) Medium only was used as a negative control to determine background fluorescence. The mpl-inferno LUT was used to depict the intensity in the mCherry channel. Scale bars represent 100 µm. CFSE, carboxyfluorescein succinimidyl ester.