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. 2021 Nov 17;13(11):1944. doi: 10.3390/pharmaceutics13111944

Figure 2.

Figure 2

Uptake of mRNA transfection complexes in the presence of colloidal gelatin. (A) Schematic overview of mRNA transfections in the presence of colloidal gelatin within a microfluidic chip. PF14 forms micelles due to its stearyl moiety, and due to its positive charge, it electrostatically complexes mRNA into polyplexes. LMM lipoplexes are pre-formed lipid micelles that complex mRNA via their cationic lipid head groups. Image not drawn to scale. (B) Confocal microscopy images of uptake into MC3T3 cells two hours post-transfection, where the Cy5-label of Cy5-eGFP mRNA is visualized. For enhanced clarity, an enlarged region of interest, which corresponds to the white rectangle (width: 736 µm, height: 209 µm) in the leftmost column, is shown in the rightmost column. The untreated conditions were used as negative controls to assess the autofluorescence of colloidal gelatin. The mpl-inferno LUT depicts the Cy5 intensity, and all Cy5 images were calibrated equally across conditions. Scale bars represent 1000 µm; scale bars in zoom-in panels represent 150 µm. Cy5, Cyanine5; NT, non-treated.