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. 2021 Nov 22;22(22):12604. doi: 10.3390/ijms222212604

Figure 2.

Figure 2

Cx46 increases the expression of CSC-related genes and CSC stemness. (A) Cancer stem cell-related genes were evaluated by measuring the mRNA levels of Oct4, Nanog, and Sox2 genes by qRT-PCR in MCF-7 and MCF-7Cx46-GFP breast cancer cells; data represent the mean of three independent experiments +/− SEM. (B) Clonogenic potential of MCF-7 and MCF-7Cx46-GFP breast cancer cells. Representative images of clonogenic assay were fixed and stained with crystal violet solution. (C) Cells were dissolved with lysis buffer and absorbance was measured at 595 nm (arbitrary units at 595 nm). The results represent the means of three independent experiments +/− SEM. (D) The graph represents the comparison of sphere diameters between MCF-7 and MCF-7Cx46-GFP before 3 weeks of culture. (E) Representative image of 3-week sphere morphology of MCF-7 and MCF-7Cx46-GFP cells; scale bar 100 μm. Data are presented as the mean of three independent experiments +/− SEM. *** p < 0.001.