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. 2021 Nov 22;22(22):12583. doi: 10.3390/ijms222212583

Figure 1.

Figure 1

Gene expression profiles and subcellular localization of OsFWL7. (A) Expression patterns of six rice FWL genes under treatment with Cd of different concentrations, as determined using RT-qPCR. The rice Actin1 gene was used for normalization of gene expression. Error bars indicate the standard deviation of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) OsFWL7 expression patterns in 14 tissue samples of Oryza sativa L. ssp. japonica variety Zhonghua 11, as determined using RT-qPCR. The tissues used were as follows: seedling, tillering, and heading stage roots (R1–R3); jointing and heading stage stems (St1 and St2); seedling, tillering, and heading stage leaves (L1–L3); 5-, 15-, and 20-cm panicles (P1–P3); and endosperms 5, 14, and 21 days after pollination (En1–En3). Error bars indicate the standard deviation of three technical replicates. (C) Subcellular localization of OsFWL7. The OsSCAMP1-mCherry construct was used as the plasma membrane marker. The GFP fluorescence, mCherry fluorescence, bright field, and merged images are shown. Bar = 10 μm.