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. 2021 Nov 22;22(22):12583. doi: 10.3390/ijms222212583

Figure 6.

Figure 6

Detection of the interactions of rice FWL proteins. (A) Yeast two-hybrid assays showing that all tested rice FWL proteins interact with themselves and one another. Transformed yeast cells were cultured on SD-Leu-Trp control medium and SD-Leu-Trp-Ade-His selective medium. Yeast strains containing the OsFWL-BD and empty pGADT7 vectors, the OsFWL-AD and empty pGBKT7 vectors, or the empty pGADT7 and pGBKT7 vectors were used as negative controls. AD, activation domain; BD, DNA binding domain. (B) Bimolecular fluorescence complementation (BiFC) assays showing self-interaction of OsFWL7 in leaf epidermal cells of Nicotiana benthamiana. The OsSCAMP1 protein was fused with mCherry and used as the plasma membrane marker. The YFP fluorescence, mCherry fluorescence, bright field, and merged images are shown. Bar = 50 μm.