Figure A1.

Phage PEV2 lysis profiles. (Curve A) Low starting MOI (=0.1): Phages must replicate and produce new virions to give rise to culture-wide bacterial lysis, as will occur after a minimum of 2 infection cycles. (Curve B) Intermediate MOI (=1): Culture-wide bacterial lysis is unlikely to be due to lysis from without (an extracellularly, enzymatically induced lysis of bacterial cells following high-multiplicity phage adsorptions), though lysis may be delayed relative to curve C due to delayed and only fractional virion adsorption. (Curve C) High starting MOI (5 or 10): Timing of bacterial lysis should occur after a single phage latent period, unless somewhat sooner due to lysis from without. For especially MOIs 5 or 10, phage infections need only retain bacteriolytic activity to result in robust drops in turbidity.