Figure 1.

Coffee pulp responses on heart and liver structure. Haematoxylin and eosin staining (×20) showing infiltration of inflammatory cells (“in”) and picrosirius red staining (×20) showing fibrosis (“fi”) in left ventricles from high-carbohydrate, high-fat diet-fed rats (C,G) compared to corn starch diet-fed rats (A,E); corn starch diet-fed rats supplemented with coffee pulp (B,F); and high-carbohydrate, high-fat diet-fed rats supplemented with coffee pulp (D,H). Haematoxylin and eosin staining (×20) showing enlarged fat vacuoles (“fv”) in livers from high-carbohydrate, high-fat diet-fed rats (K) compared to corn starch diet-fed rats (I), corn starch diet-fed rats supplemented with coffee pulp (J) and high-carbohydrate, high-fat diet-fed rats supplemented with coffee pulp (L).