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. 2021 Nov 9;22(22):12092. doi: 10.3390/ijms222212092

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of DELLA proteins in flowering Chinese cabbage. (A) Conserved domain of the DELLA genes. Green and yellow represent the DELLA and GRAS domain, respectively. (B) Conserved motifs of the DELLA genes. Different motifs are represented by different colored boxes with numbers 1−10. (C) Phylogenetic tree of DELLA proteins in flowering Chinese cabbage and other species, as determined by the neighbor-joining method. The protein IDs used in the phylogenetic tree are AtRGA1 (NP_178266.1), AtGAI (NP_172945.1), AtRGL1 (NP_176809.1), AtRGL2 (NP_186995.1), AtRGL3 (NP_197251.1), BrRGA1 (XP_009101333.1), BrRGA2 (XP_009114228.2), BrRGL1 (XP_009127484.1), BrRGL2 (XP_009130367.1), BrRGL3 (XP_009107522.1), SlGAI (NP_001234365.1), D8 (NP_001354393.1), OsGAI (XP_015631543.1), and VvGAI (XP_002284648.1). (D) Detection of interactions among DELLA proteins and BcGID1b using Y2H. Yeast cells with different construct combinations were grown on selective media without Trp and Leu (-TL) and were tested for interactions on selective media without Trp, Leu, His, and Ade (−TLHA). (E) Expression profiles of flowering Chinese cabbage DELLA genes in various organs. Above the line is a horizontal comparison, with each column exhibiting normalization independently, and below the line is a vertical comparison, with each row displaying normalization independently. GA₃, gibberellin A3.