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. 2021 Nov 12;22(22):12232. doi: 10.3390/ijms222212232

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Angptl2 expression in human senescent endothelial cells. (A) Senescence-associated β-galactosidase staining of senescent (left panel) compared to non-senescent (right panel) cultured hIMAEC; same magnification ×400 (adapted from [61]). (B) Immunofluorescence images of senescent (top panel) compared to non-senescent (bottom panel) cultured hIMAEC for angptl2 (adapted from [53]), the oxidative stress marker HNE (adapted from [61]), the DNA damage marker ATM (adapted from [62]) and the senescent marker p21 (NTT, unpublished data). ATM: ataxia-telangiectasia mutated; hIMAEC: human internal mammary artery endothelial cells; HNE: hydroxy-nonenal.