Table 1.
Predicted CTL epitopes. The selection of appropriate candidates was based on C-terminal cleavage, TAP scores, and antigenicity. The CTL epitopes shown in bold are the finally selected epitopes for multi-epitopes vaccine construct.
| Residue No. | Peptide Sequence | MHC Binding Affinity | Rescale Binding Affinity | C-Terminal Cleavage Affinity | Transport Affinity | Prediction Score | MHC-I Binding | Antigenecity Score |
|---|---|---|---|---|---|---|---|---|
| 2237 | SSDDNKLAY | 0.7693 | 3.2662 | 0.9150 | 2.9740 | 3.5521 | YES | Non-antigenic |
| 184 | NVDGVYLEY | 0.6968 | 2.9587 | 0.9788 | 3.0560 | 3.2583 | YES | 0.876 |
| 3035 | STLNGGLFY | 0.6183 | 2.6251 | 0.9456 | 3.0220 | 2.9180 | YES | Non-antigenic |
| 98 | ATDWMSWLL | 0.5851 | 2.4842 | 0.9229 | 0.9550 | 2.6704 | YES | Non-antigenic |
| 1788 | SIAARGHLY | 0.4418 | 1.8759 | 0.9337 | 3.1000 | 2.1710 | YES | 0.524 |
| 3262 | ETACLSKAY | 0.4369 | 1.8550 | 0.2200 | 2.8480 | 2.0304 | YES | 0.691 |
| 2130 | CVSALDVFY | 0.4011 | 1.7029 | 0.7949 | 3.1500 | 1.9797 | YES | Non-antigenic |
| 3018 | GVEGISLNY | 0.3920 | 1.6642 | 0.9715 | 2.8940 | 1.9546 | YES | 1.4 |
| 451 | KTILTMGEY | 0.3915 | 1.6621 | 0.7760 | 2.9120 | 1.9241 | YES | 0.494 |
| 422 | KVEPHTGDY | 0.3583 | 1.5213 | 0.9717 | 3.0730 | 1.8207 | YES | 0.707 |
| 971 | HTDQSLWMR | 0.3781 | 1.6055 | 0.7247 | 1.2050 | 1.7744 | YES | Non-antigenic |
| 3077 | ATTIMQKAY | 0.3578 | 1.5192 | 0.4362 | 3.0590 | 1.7375 | YES | 0.454 |
| 1955 | RQDGRTDEY | 0.3297 | 1.3998 | 0.9504 | 3.0380 | 1.6942 | YES | Non-antigenic |
| 800 | EVSEWYDNY | 0.3269 | 1.3879 | 0.9476 | 2.9870 | 1.6794 | YES | Non-antigenic |
| 2530 | CTREEFFVY | 0.3072 | 1.3045 | 0.9681 | 3.0520 | 1.6023 | YES | 0.617 |
| 1783 | WTDPHSIAA | 0.3502 | 1.4869 | 0.4722 | 0.7830 | 1.5185 | YES | Non-antigenic |
| 2214 | MAGVALIFY | 0.2864 | 1.2161 | 0.5939 | 3.0020 | 1.4553 | YES | 0.532 |
| 2855 | MTDTTAFGQ | 0.3395 | 1.4416 | 0.0703 | 0.1020 | 1.4470 | YES | Non-antigenic |
| 2813 | RTWQYWGSY | 0.2555 | 1.0848 | 0.9775 | 3.2670 | 1.3948 | YES | Non-antigenic |
| 3342 | VMEWRDVPY | 0.2301 | 0.9770 | 0.9072 | 3.0960 | 1.2679 | YES | 1.79 |
| 980 | SMKNDTGTY | 0.2236 | 0.9492 | 0.9495 | 3.2290 | 1.2530 | YES | Non-antigenic |
| 1143 | VALFVVLEY | 0.2002 | 0.8501 | 0.9747 | 3.0660 | 1.1496 | YES | 0.652 |
| 1235 | VTTYFLLLV | 0.2186 | 0.9279 | 0.7355 | 0.3550 | 1.0560 | YES | Non-antigenic |
| 598 | PTDSGHDTV | 0.2370 | 1.0062 | 0.3920 | 0.2090 | 1.0545 | YES | 0.425 |
| 553 | VAHIEGTKY | 0.1785 | 0.7579 | 0.9739 | 2.9240 | 1.0502 | YES | Non-antigenic |
| 2133 | ALDVFYTLM | 0.2087 | 0.8861 | 0.9760 | 0.3460 | 1.0498 | YES | Non-antigenic |
| 1634 | AQGVVVGLY | 0.1806 | 0.7667 | 0.8269 | 3.1220 | 1.0469 | YES | 0.718 |
| 1677 | WTSKGQITV | 0.2126 | 0.9025 | 0.8831 | 0.2320 | 1.0466 | YES | 1.19 |
| 448 | SSEKTILTM | 0.2101 | 0.8922 | 0.9417 | 0.2630 | 1.0466 | YES | Non-antigenic |
| 411 | VYDANKIVY | 0.1765 | 0.7494 | 0.8988 | 3.0520 | 1.0369 | YES | Non-antigenic |
| 994 | VTDLRNCSW | 0.1987 | 0.8437 | 0.9180 | 0.7970 | 1.0212 | YES | 1.377 |