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. 2021 Nov 24;221(2):e202107070. doi: 10.1083/jcb.202107070

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© 2021 Crosby et al.

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Figure 2. — ATL1 and ATL2 are autoinhibited by a variable C-terminal extension. (A) Alignment of ATL1 and ATL2 N-terminal extensions. (B) Lipid mixing by full-length (FL) ATL1, ΔN ATL1(30–558), FL ATL2, and ΔN ATL2(56–583). (C) Alignment of ATL1 and ATL2 C-terminal extensions. (D) Lipid mixing by FL ATL1, ΔC ATL1(1–520), FL ATL2, and ΔC ATL2(1–547). (E) Lipid mixing between FL and DC ATL2 in trans. DC ATL2 in labeled liposomes (L) were mixed with FL ATL2 in unlabeled liposomes (UL) at a 1:2 ratio or vice versa. (F) Schematic of ATL1/2 chimeras. (G) Lipid mixing by ATL1 WT, ΔC ATL1, and ATL1-ATL2 C-terminal chimera. (H) Lipid mixing by ATL2 WT, ΔC ATL2, and ATL2-ATL1 C-terminal chimera. All proteins were incorporated at a 1:1,000 protein/lipid ratio, and the data are the average of at least two independent traces.