Figure 5.

ATL2(1–547) catalyzes full fusion. (A) ATL2(1–547) catalyzes both outer and inner leaflet lipid mixing. Inner leaflet mixing was measured after first quenching NBD in the outer leaflet with the membrane-impermeable compound sodium dithionite. For reference, the data are plotted relative to total lipid mixing by the same variant. (B) Dynamic light scattering of FL ATL2 liposomes (left) or ATL2(1–547) liposomes (right) incubated at 37°C with buffer (upper panels, no nuc), 2 mM GTP for 10 min (middle panels), or 2 mM GTP for 10 min followed by 10-min EDTA treatment (18 mM final concentration).