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. 2021 Nov 24;221(2):e202107070. doi: 10.1083/jcb.202107070

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© 2021 Crosby et al.

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Figure 6. — Cryo-ET analysis of tethering and fusion. FL ATL2 and ATL2(1–547) were reconstituted into liposomes, incubated with GTP for 30 s, and processed for cryo-EM. (A) FL ATL2 liposomes remained at the starting size of 100–300 nm, while ATL2(1–547) liposomes were substantially larger. (B) FL ATL2 liposomes were predominantly tethered by tight zippers (white arrows), whereas ATL2(1–547) liposomes appeared to be attached by both tight zippers (white arrows) and more diffuse zippers (yellow arrows). Scale bars, 100 nm.