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. 2021 Nov 24;221(2):e202107070. doi: 10.1083/jcb.202107070

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© 2021 Crosby et al.

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Figure S1. — Phosphorylation does not account for ATL1 fusion activity. (A) Intensity of singly and doubly phosphorylated TYEWSSEEEEPVKK peptides and the unmodified counterpart. (B) Lipid mixing by WT ATL1 and phosphomutant S22A, S23A. (C) Lipid mixing by DATL purified from HEK cells is faster than by DATL purified from E. coli. All lipid mixing was performed at a 1:1,000 protein/lipid ratio, and the data are the average of at least two individual traces.